Liquid NMR Services

About Liquid-State NMR Spectroscopy

The vast majority of NMR samples are studied in the liquid-state where solid samples are dissolved in the appropriate deuterated solvent. In order to study a sample by liquid-state NMR spectroscopy:

  • Enough sample must be provided to make a 0.6 mL solution of at least 1mM. Usually, more sample is required and the solution concentration required will depend on the desired NMR nucleus of interest and experiment. In most cases, solutions of at least 10mM are preferred. If your sample is not appreciably soluble in any known solvent, you may consider using solid-state NMR spectroscopy instead.

  • The sample should be diamagnetic. Paramagnetic samples can be studied by NMR, but the NMR is much more complicated and may not give rise to any useable data.

Services Provided

Depending on your experience using NMR spectroscopy as a means of structural characterization, the NMR facility can perform a variety of functions. For experienced users, staff generally just acquire the NMR data and provide the raw NMR data. For novice users, the NMR facility can prepare the NMR sample, acquire the NMR data, process, and completely analyze the NMR spectra. For all external service work, a report summarizing the NMR experiments performed and the experimental conditions utilized are included. Experiments are typically completed within 48 hours of sample receipt. If the complete analysis is required, at least 1 week is necessary.

The NMR facility provides a base service for all samples, which includes:

  • NMR tube
  • NMR solvent (deuterated solvent)
  • preparation of solution for NMR
  • acquisition of a 1D 1H NMR spectrum at 25 oC
  • e-mailing of raw NMR data (FID)
  • service summary report outlining the NMR experiments performed, NMR conditions and charges (external samples only)
  • sample disposal

For an additional charge above that of the base service, the NMR facility can perform the following NMR experiments:

  • Standard direct-detect 1D NMR experiments on 13C, 19F, and 31P.
  • Odd-nuclei direct-detect 1D NMR experiments on nuclear isotopes such as 2H, 11B, 77Se. Many other nuclear isotopes can be studied as well. Please enquire.
  • Indirect-detect heteronuclear 2D NMR experiments on nuclear isotopes such as 15N, 29Si. Many other nuclear isotopes can be studied as well. Please enquire.
  • Homonuclear 2D 1H-1H NMR experiments such as COSY, TOCSY, NOESY/ROESY.
  • Any of the above experiments can also be performed above or below room temperature.

For an additional charge above that of the base service, the NMR facility will perform or help with the following tasks:

  • Processing of the NMR data into an NMR spectrum.
  • Analysis of the NMR spectra, including integration, peak picking, and peak assignments where applicable.
  • Interpretation of 1D and 2D NMR spectra.

External Service Rates

The rates for NMR service for non-Western clients can be found in the table below.  If desired, one can also download the Liquids NMR Service Rates in a PDF file.

Rates for Liquids NMR Service

User Rates for Liquid NRM Services
Rates for Liquids NMR Service
Experiment Academia /
Not for Profits
Industry Comments
Base Service $30 $50 Base service includes:
  • NMR solventa
  • NMR tube
  • preparation of NMR solution
  • acquisition of 1H NMR spectrum at 25 °C
  • e-mailing of raw 1H NMR data (the 1H NMR FID)
  • service summary report (external samples only)
  • sample disposal
+Additional Charges (for any additional experiments, processing or analysis above the base service)

Processing and Analysis Rates

User Rates for Liquid NRM Services
Processing and Analysis
Experiment Academia /
Not for Profits
Industry Comments
Basic analysis $15 $25 Includes electronic PDF plots with peak picking and integration (if applicable).
Interpretation $50/hour $100/hour Charge for NMR facility staff to provide an interpretation of the NMR spectra, spectral assignments and a written report explaining the interpretation.

Variable-Temperature Rates

User Rates for Liquid NRM Services
Variable-Temperature
Experiment Academia /
Not for Profits
Industry Comments
> 25 °C $15 $25 Charge per temperature point.  The maximum temperature is +120 °C.
< 25 °C $25 $35 Charge per temperature point.  The minimum temperature is −80 °C.

1D NMR Experiments Ratesb

User Rates for Liquid NRM Services
1D NMR Experimentsb
Experiment Academia /
Not for Profits
Industry Comments
1H $10 $15 For each additional 1H spectrum acquired above or below room temperature.
13C $20 $30 Charge per experiment per temperature in addition to the base service price.
19F $10 $15 Charge per experiment per temperature in addition to the base service price.
31P $15 $20 Charge per experiment per temperature in addition to the base service price.
{1H,1H} Selective $15 $20 Includes 1D TOCSY, NOESY or ROESY. Charge per irradiation point per temperature in addition to the base service price.

2D NMR Experiments Ratesb

User Rates for Liquid NRM Services
Homonuclear 2D NMR Experiments
Experiment Academia /
Not for Profits
Industry Comments
{1H,1H} gCOSY $15 $25 Charge per experiment per temperature in addition to the base service price.
{1H,1H} TOCSY $20 $35 Charge per experiment per temperature in addition to the base service price.
{1H,1H} NOESY $50 $100 Same price for a ROESY. Charge per experiment per temperature in addition to the base service price.
Heteronuclear 2D NMR Experiments
{1H,1C} gHSQC $20 $35 Charge per experiment per temperature in addition to the base service price.
{1H,1C} gHMBC $30 $55 Charge per experiment per temperature in addition to the base service price.

Solvents are between 99.5% to 99.9%-d and are stored in individually sealed ampoules. The price quoted applies only to acetone-d6, benzene-d6, CDCl3, DMSO-d6, and D2O. Prices will be provided upon request for higher deuteration or other solvents such as acetonitrile-d3, CD2Cl2, methanol-d4, THF-d8, etc..

NMR experiments can be performed on nuclei other than those listed in the table; prices will be provided upon request.

Sample Submission Procedures

For Western Personnel

The following procedure should be used when submitting a sample for service work:

  1. Contact NMR facility staff via e-mail at chemnmr@uwo.ca or phone at 519-661-2111 x. 82905 to discuss what information you are looking to obtain by using NMR, what solvent should be used, the nature of your sample, and what services are required.

  2. Fill out and print the Liquids NMR Service Request - Western Personnel form. Note that the form is a fillable PDF form that can be filled out electronically.

  3. If you are preparing your own NMR sample, bring the NMR sample and the completed sample submission form to the NMR facility. If you are not preparing your own NMR sample, bring at least 20mg of sample in a screw-top vial and the completed sample submission form to the NMR facility.

  4. Requested data will be sent via e-mail generally within 48 hours of sample receipt.

For External Clients (Non-Western Personnel)

The following procedure should be used when submitting a sample for service work:

  1. Contact NMR facility staff via e-mail at chemnmr@uwo.ca or phone at 519-661-2111 x. 82905 to discuss what information you are looking to obtain by using NMR, what solvent should be used, the nature of your sample and what services are required.

  2. Fill out and print the Liquids NMR Service Request - External Clients form. Note that the form is a fillable PDF form that can be filled out electronically.

  3. If you are preparing your own NMR sample, bring the NMR sample and the completed sample submission form to the NMR facility. If you are not preparing your own NMR sample, bring at least 20mg of sample in a screw-top vial and the completed sample submission form to the NMR facility. If you are off-campus, the sample can be mailed, along with the completed sample submission form to:

Mathew Willans
Western University
1151 Richmond St. N.
Department of Chemistry
Dock 003, Room 11
London, ON
N6A 5B7

  1. Requested data will be sent via e-mail generally within 48 hours of sample receipt. Depending on the analysis required, the sample report will generally be sent via e-mail within 7 days after all NMR spectra have been acquired.