Associate Professor
Department of Biology
Western University
1151 Richmond St. N
London, Ontario, Canada
N6A 5B7
Office: Biological & Geological Sciences 3032
Email: atimoshe@uwo.ca
Phone: (519) 661-2111 x 88900
Fax: 519 661-3935
Biology 3326F/G Cell Biology Laboratory
This is a laboratory course which provides training in current cell biological methods such as tissue culture, microscopy, protein and genomic DNA isolation, PCR, SDS-PAGE, fluorescence staining of cellular organelles, immunostaining, and bioimaging. An important part of this course focuses on application of the mentioned methods to study protein expression and cytoskeleton organization in cultured animal cells and to analyze the cytoskeleton rearrangements in response to stress. Students will be actively engaged in scientific writing and critical thinking by reporting their experimental results in a form of research paper and poster presentation.
Biology 3355A Molecular Cell Biology of Stress
The course covers recent research findings in the area of environmental, physiological, and pathological stress in animal systems. The focus is on both the evolutionary conserved aspects of cell stress responses (e.g. heat shock proteins and chaperones) and on individual signaling pathways and molecules controlling the action of specific stress stimuli. The main lecture topics include heat shock response, endoplasmic reticulum stress and unfolded protein responses, oxidative stress, hypoxic stress, osmotic stress, inflammatory stress, DNA damage response, microRNA in stress responses, nucleolar stress, glycobiology of stress. There will be several guest lectures of experts in the field of cell stress biology. Students are required to give a powerpoint presentation of a primary research paper chosen from peer-reviewed scientific journals (tutorial component).
Biology 4999E Honors Research Thesis
Supervisor and Advisor of individual research projects.
Biology 4970F/G Independent Study in Biology
Supervisor and Advisor of individual research projects.