Jim StaplesWestern Science


2011 - Present | 1995-2010

Publications 2011 - Present

Wang, X., Staples, J.F., Watt, C.F., McKenzie and C.A. MacCannell, A.D.V. 2019. Thermoneutral temperature reduces liver volume but increases fat content in a mammalian hibernator. J. Therm. Biol. 83: 172-177: doi 10.1016/j.jtherbio.2019.05.015

Mathers, K.E.* and Staples, J.F. Differential post-translational modification of mitochondrial enzymes corresponds with metabolic suppression during hibernation. in press, Am. J. Physiol. R. 317:R262-R269 doi.org/10.1152/ajpregu.00052.2019. Chosen as APSselect for "outstanding scientific discoveries".

MacCannell, A.D.V.*, Sinclair K.F., Tattersall, G., McKenzie, C.A., and Staples, J.F. 2019. Identification of a lipid-rich depot in the orbital cavity of the 13-lined ground squirrel. in press, J. Exp. Biol. 222. doi:10.1242/jeb.195750. Featured in "Inside JEB"

MacCannell, A.D.V., Sinclair K.F., Staples, J.F. and McKenzie, C.A. 2019. Environmental temperature effects on adipose tissue growth in a hibernator. J. Exp. Biol.,

MacCannell, A.D.V., Jackson, E.C., Mathers, K.E. and Staples, J.F. 2018. An improved method for detecting entrance and arousal in a mammalian hibernator using heart rate data. J. Exp. Biol. in press,

Dugbarey, G.J., Hardenberg, M.C., Kok, W.F., Boerema, A.S., Carey, H.V., Staples, J.F., Henning, R.F. and Bouma, H.R. 2017. Renal mitochondrial response to low temperature in non-hibernating and hibernating species. Antioxidants and Redox Signalling. 27:599-617.

MacCannell, A., Sinclair, K., Friesen-Waldner, L. McKenzie, C. and Staples, J. 2017. Water-fat MRI reveals growth of brown adipose tissue without cold exposure in a hibernator. in press. J. Comp. Physiol. 187:759-767 DOI: 10.1007/s00360-017-1075-8

McFarlane, S.V., Mathers, K.E. and Staples, J.F. 2017. Reversible temperature-dependent alterations of brown adipose tissue mitochondrial respiration during torpor in a mammalian hibernator.

Mathers, K.E., McFarlane, S.V., Zhao, L. and Staples, J.F. 2017. Regulation of mitochondrial metabolism during hibernation by reversible suppression of electron transport system enzymes. J. Comp. Physiol. B 187:227–234. DOI 10.1007/s00360-016-1022-0

Robertson, A., Schaltz, K., Neimanis, K., Staples, J.F. and McDonald*, A.E. 2016. Heterologous Expression of the Crassostrea gigas (Pacific Oyster) Alternative Oxidase in the Yeast Saccharomyces cerevisiae. J. Bioenerget. Biomembr. 48:509-520. DOI:10.1007/s10863-016-9685-5.

Staples J.F. 2016. Metabolic Flexibility: Hibernation, Torpor and Estivation. invited review Comprehensive Physiol. 6(2): 737-771.

Mathers, K.E. and Staples, J.F. 2015. Saponin-permeabilization is not a viable alternative to isolated mitochondria for assessing oxidative metabolism in hibernation. Biol. Open. 4:858-864. DOI - 10.1242/​bio.011544.

Dunlop, K., Cedrone, M., Staples, J.F and Regnault, T.R.H. 2015. Altered Fetal Skeletal Muscle Nutrient Metabolism Following an Adverse In Utero Environment and the Modulation of Later Life Insulin Sensitivity. Nutrients. 7: 1202-1216.

MacMillan H.A., Ferguson, L.V., Nicolai, A. Donini, A., Staples, J.F. and Sinclair, B.J. 2015. Parallel ionoregulatory adjustments underlie phenotypic plasticity and evolution of Drosophila cold tolerance. J. Exp. Biol. 218: 423-432.

Staples, J.F. 2014. Metabolic Suppression in Mammalian Hibernation: The Role of Mitochondria. J. Exp. Biol. 217:2032-2036. doi:10.1242/jeb.092973

Brown, J.C.L. and Staples, J.F. 2014. Substrate-specific suppression of mitochondrial subpopulations in skeletal and cardiac muscle of hibernating thirteen-lined ground squirrels. in press, J. Comp. Physiol B. 184(3):401-414. DOI  - 10.1007/s00360-013-0799-3.

Cooper, A.N., Brown, J.C.L. and Staples, J.F. 2014. Are long chain acyl CoAs responsible for suppression of mitochondrial metabolism in hibernating 13-lined ground squirrels. Comp. Biochem. Physiol. B. 170:50-57. DOI - 10.1016/j.cbpb.2014.02.002

Neimanis, K., Staples, J.F. Hüner, N.P.A. and McDonald, A.E. 2013. Identification, Expression, and Taxonomic Distribution of Alternative Oxidases in Non-Angiosperm Plants. Gene 526:275-286.

Chung, D.C., Szyszka, B., Brown, J.C.L., Hüner, N.P.A. and Staples, J.F. 2013. Changes in the mitochondrial phosphoproteome during mammalian hibernation. Physiol. Genom. 45(10):389-399.

James, R.S., Staples, J.F., Brown, J.C., Tessier, S.N. and Storey, K.B. 2013. The effects of hibernation on the contractile and biochemical properties of skeletal muscles in the thirteen-lined ground squirrel, Ictidomys tridecemlineatus. J. Exp. Biol. 216:2587-2594.

Suri, L.M.N., Cruz, A., McCaig, L., Veldhuizen, R.A.W., Staples, J.F., Possmayer, F. Orgeig, S., Perez-Gil, P. 2013. Adaptations to hibernation in lung surfactant composition of 13-lined ground squirrels influence surfactant lipid phase segregation properties. Biochim. Biophys. Acta. 1828(8):1707-1714.

Brown J.C.L., Chung D.J., Cooper A.N., Staples J.F. 2013. Regulation of succinate-fuelled mitochondrial respiration in liver and skeletal muscle of hibernating thirteen-lined ground squirrels. J. Exp. Biol. 216:1736-1743.

Price, E.R., C. Armstrong, C, Guglielmo, C.G. and Staples, J.F. 2013. Selective mobilization of saturated fatty acids in isolated adipocytes of hibernating thirteen-lined ground squirrels Ictidomys tridecemlineatus. Physiol. Biochem. Zool. 86(2):205-212.

Gallagher, K. and Staples, J.F. 2013. Metabolism of brain cortex and cardiac muscle mitochondria in hibernating thirteen-lined ground squirrels (Ictidomys tridecemlineatus). Physiol. Biochem. Zool. 86(1):1-8.

MacMillan, H.A, Williams, C.M., Staples, J.F. and Sinclair, B.J. 2012. Reestablishment of ion homeostasis during chill-coma recovery in the cricket Gryllus pennsylvanicus. Proc. Nat. Acad. Sci. 109(50):20750-20755.

Staples, J.F. Fast In, Slow Out: Patterns and Mechanisms of Mitochondrial Metabolic Suppression in Mammalian Hibernation. In: Ruf T, Bieber C, Arnold W, Millesi E (eds). 2012. Living in a seasonal world: thermoregulatory and metabolic adaptations. Pp. 377-386. Springer, Heidelberg.

Brown, J, Marshall, K., Staples, J. 2012. Differences in tissue concentrations of hydrogen peroxide in the roots and cotyledons of annual and perennial species of flax (Linum). Botany. 90(10): 1015-1027

Suri L., McCaig L., Picardi, M., Ospina, O., Veldhuizen, R., Staples, J., Possmayer, F., Yao, L., Perez-Gil, J., Orgeig, S. 2012. Adaptation to low body temperature influences pulmonary surfactant composition thereby increasing fluidity while maintaining appropriately ordered membrane structure and surface activity. BBA-Biomembranes. 1818:1581-1589.

MacMillan, H.A, Williams, C.M., Staples, J.F. and Sinclair, B.J. 2012. Metabolism and energy supply below the critical thermal minimum of a chill-susceptible insect. J. Exp. Biol. 215:1366-1372.

Brown, J.C.L., Chung, D.J., Belgrave, K.R. and Staples, J.F. Mitochondrial metabolic suppression and reactive oxygen species production in liver and skeletal muscle of hibernating thirteen-lined ground squirrels. 2012. Am. J. Physiol. 302:R15-R28.

Staples, J.F. Maintaining metabolic balance in mammalian hibernation and daily torpor. 2011. in Nowakowska, A. and M. Caputa (eds.) Hypometabolism: strategies of survival in vertebrates and invertebrates. Research Signpost, Kerala, India. pp. 95-115.

Brown, J.C.L. and Staples, J.F. 2011. Suppression of mitochondrial metabolism during daily torpor and fasting in house mice (Mus musculus) and dwarf Siberian hamsters (Phodopus sungorus): consequences for reactive oxygen species release. Physiol. Biochem. Zool. 84:467-480.

Armstrong C., Thomas, R., Price, E.R., Guglielmo, C. G. and Staples, J. F. 2011. Remodeling mitochondrial membrane during arousal from hibernation. Physiol. Biochem. Zool. 84:438-449.

Chung, D., Lloyd, G.P., Thomas, R.H., Guglielmo, C.G. and Staples, J.F. 2011. Mitochondrial respiration and succinate dehydrogenase are suppressed early during entrance into a hibernation bout, but membrane remodeling is only transient. J. Comp. Physiol. B 181(5):699-711.

Price, E.R., Staples, J.F., Milligan, C.L. and Guglielmo, C.G. 2011. Carnitine palmitoyl transferase activity and whole muscle oxidation rates vary with fatty acid substrate in avian flight muscles. J. Comp. Physiol. B. 181(4):565-573.

Publications 1995-2010

Armstrong, C. and Staples, J.F. 2010. The role of succinate dehydrogenase and oxaloacetate in mitochondrial metabolism during mammalian hibernation and arousal. J. Comp. Physiol. B. 180:775-783.

Brown, J.C.L. and Staples, J.F. 2010. Mitochondrial metabolism during fasting-induced daily torpor in mice (Mus musculus). Biochim. Biophys.
Acta-Bioenerg. 1797(4):476-486.

Brown, J.C.L., McClelland, G.B., Faure, P.A., Klaiman, J.M. and Staples,
Mechanisms of reduced ROS production in long-lived species. 2009.
Mech. Ageing Dev. 130:467-476.

McDonald, A.E., Vanlerberghe, G.C. and Staples, J.F. Alternative oxidase
in animals: Unique characteristics and taxonomic distribution. 2009. J.
Exp. Biol. 212:2627-2634

Staples, J.F. and Buck, L.T. 2009. Matching metabolic supply and demand in energy-stressed animals. Comp. Biochem. Physiol. A. 153:95-105.

Page, M.M., C. Peters, J.F. Staples and Stuart J.A. 2009. Intracellular superoxide dismutases are not globally upregulated during hibernation in the major oxidative tissues in the 13-lined ground squirrel Spermophilus tridecemlineatus. Comp. Biochem. Physiol. A 152:115-122.

Staples J.F. and J.C.L. Brown. 2008. Mitochondrial metabolism in hibernation and daily torpor: a review. J. Comp. Physiol. B. 178:811-827.

Gerson, A.R., J.C.L. Brown, R. Thomas, M.A. Bernards and J.F. Staples.
The effect of dietary polyunsaturates on mitochondrial metabolism in hibernation. J. Exp. Biol. 211: 2689-2699.

Staples, J.F., Kajimura, M., Wood, C.M., Patel, M., Ip, Y.K., and McClelland, G.B. (2008) Enzymatic and mitochondrial responses to 5 months of aerial exposure in the slender lungfish (Protopterus dolloi). J. Fish Biol.73:608-622.

Ellard, J.P., McCudden, C.R. Tanega, C. James, K.A., Ratkovic, S., Staples, J.F., Wagner, G.F. 2007. The respiratory effects of stanniocalcin-1 (STC-1) on intact mitochondria and cells: STC-1 uncouples oxidative phosphorylation and its actions are modulated by nucleotide triphosphates. Mol. Cell. Endocrinol. 264:90-101.

Brown, J.C.L., A.R. Gerson, J.F. Staples. 2007. Mitochondrial metabolism during daily torpor in the dwarf Siberian hamster: the role of active regulated changes and passive thermal effects. Am. J. Physiol. 293:R1833-1845.

Muleme, H. M, Walpole, A. C. and Staples, J. F. 2006. Mitochondrial metabolism in hibernation: Metabolic suppression, temperature effects and substrate preferences. Physiol. Biochem. Zool. 79(3): 474-483.

West, T.G., Donohoe, P.H., Staples, J.F. and Askew, G.N. 2006. The role of skeletal muscle in the hypoxia induced responses of submerged frogs., J. Exp. Biol. 209:1159-1168.

Schaefer, C. D. and Staples, J. F. 2006. Mitochondrial metabolism in mammalian cold acclimation: magnitude and mechanisms of fatty acid induced uncoupling. J. Therm. Biol. 31(4):355-361.

Staples, J. F., Koen, E.L. and Laverty, T.L. 2004. “Futile cycle” enzymes in the flight muscles of North American bumblebees. J. Exp. Biol. 207(5): 749-754.

Staples, J. F., Webber, D. M. and Boutilier, R. G. 2003. Environmental hypoxia does not constrain the diurnal depth distribution of free-swimming Nautilus pompilius. Physiol. Biochem. Zool. 76(5):644-651.

Staples, J. F., Hershkowitz, J. J. and Boutilier, R. G. (2000). Effects of ambient PO2 and temperature on oxygen uptake in Nautilus pompilius. J. Comp. Physiol. B. 170(3):231-236.

Suarez, R. K., Staples, J. F., Lighton, J. R. B. and Mathieu-Costello, O (2000). Mitochondrial function in flying honeybees (Apis mellifera): respiratory chain enzymes and electron flow from complex III to oxygen. J. Exp. Biol. 203:905-911.

Suarez, R. K., Staples, J. F. and Lighton, J. R. B. (1999). Turnover rate of mitochondrial respiratory chain enzymes in flying honeybees (Apis mellifera). J. Exp. Zool. 284(1):1-6.

Staples, J. F. and Hochachka, P. W. (1998). The effect of hibernation status and cold-acclimation on hepatocyte gluconeogenesis in the golden-mantled ground squirrel (Spermophilus lateralis). Can. J. Zool. 76(9):1734-1740.

Hochachka, P. W., McClelland, G.B., Burness, G.P., Staples, J.F. and Suarez, R.K. (1998) Integrating metabolic pathway fluxes with gene-to-enzyme expression rates. Comp. Biochem. Physiol. B. 120(1):17-26.

Staples, J. F. and Suarez, R. K. (1997). Honeybee flight muscle phosphoglucose isomerase: Matching enzyme capacities to flux requirements at a near-equilibrium reaction. J. Exp. Biol. 200:1247-1254.

Staples, J. F. and Hochachka, P. W. (1997). Liver energy metabolism during hibernation in the golden-mantled ground squirrel, Spermophilus lateralis. Can. J. Zool. 74(7):1059-1065.

Suarez, R. K., Staples, J. F., Lighton, J. R. B. and West, T. G. (1997). Relationships between enzymatic flux capacities and metabolic flux rates: Nonequilibrium reactions in muscle glycolysis. Proc. Natl. Acad. Sci. 94:7065-7069.