Susanne KohalmiWestern Science

Recent Publications

Flower
[photo: Susanne Kohalmi]

Clayton EJ*, Smith-Uffen M*, Duennwald ML, Kohalmi SE. (2025). Details matter: Defining substrate specificity in arogenate versus prephenate dehydratases. BMC Biology accepted with revisions; doi: 10.21203/rs.3.rs-5357451/v1

Sakariyahu KS, McDowell T , Renaud JB, Papadopoulos Y, Glover K, a Nelson Brown R, Peel MD, Riday H, Kohalmi SE, Hannoufa A 2025. Profiling environmental variations in condensed tannins and other metabolites of birdsfoot trefoil (Lotus corniculatus) genotypes. Plants 14, 2766, doi.org/10.3390/plants14172766

Chaplin A, Kohalmi SE, Simon A. (2024). Impact of the COVID-19 pandemic on undergraduate research in the department of biology at Western University: Effect on project types, learning outcomes, and student perceptions. bioRxiv doi: https://doi.org/10.1101/2024.01.24.577125.

Clayton EJ, Islam NS, Pannunzio K, Kuflu K, Sirjani R, Kohalmi SE, Dhaubhadel S 2024. Soybean AROGENATE DEHYDRATASES (GmADTs) and isoflavonoid biosynthesis: involvement in the cytosolic isoflavonoid metabolon or transorganelle continuity? Frontiers in Plant Science. Special Issue on Plant Metabolism and Chemodiversity. doi: 10.3389/fpls.2024.1307489

Nasrollahi V, Allam G, Kohalmi SE, Hannoufa A 2023. MsSPL9 modulates nodulation under nitrate sufficiency condition in Medicago sativa. International Journal of Molecular Sciences (special issue: Abiotic stresses in plants: From molecules to environment) 24 9615, doi: 10.3390/ijms24119615

VanderBurgt JT, Harper O, Garnham C, Kohalmi SE Menassa R 2023. Plant production of a virus-like particle-based vaccine candidate against porcine reproductive and respiratory syndrome. Frontiers in Plant Science, section Plant Biotechnology 14 1044675. doi: 10.3389/fpls.2023.1044675

Saatian B, Kohalmi SE, Cui Y 2023. Localization of Arabidopsis glucan synthase-like 5, 8 and 12 to plasmodesmata and the GSL8-dependent role of PDLP5 in regulating plasmodesmal permeability. Plant Signaling and Behavior 18 216470. doi: 10.1080/15592324.2022.2164670

Nasrollahi V,Yuan Z-C, Lu QSM, McDowell T, Kohalmi SE, Hannoufa A 2022 Deciphering the role of SPL12 and AGL6 from a genetic module that functions in nodulation and root regeneration in Medicago sativa. Plant Molecular Biology 110 511-529.

Thapa RK, Tian G, Xie X, Kohalmi SE, Cui Y 2022. Involvement of NUCLEOPORIN1 in cell division and expansion in Arabidopsis. Plant Gene 32,100385

Nasrollahi V, Yuan Z-C, Kohalmi SE, Hannoufa A 2022. SPL12 regulates AGL6 and AGL21 to modulate nodulation and root regeneration under osmotic stress and nitrate sufficiency conditions in Medicago sativa. Plants 11, 3071.
Simkovich A, Kohalmi SE, Wang A.2022. Purification and proteomics analysis of phloem tissues from virus-infected plants. Methods in Molecular Biology 2400, 125-137.

Simkovich AJ, Li Y, Kohalmi SE, Griffiths JS, Wang A 2021 Molecular identification of prune dwarf virus (PDV) infecting sweet cherry in Canada and development of a PDV full-length infectious cDNA clone. Viruses. 13, 2025.

Simkovich AJ, Kohalmi SE, Wang A 2021. First report of little cherry virus 1 infecting sweet cherry in Ontario, Canada. Plant Disease 105, 44173.

Simokovich AJ, Kohalmi SE, Wang A 2021. First report of Cherry virus A infecting sweet cherry in Ontario, Canada. New Disease Reports 44, e12022 (open access).

Song J, Xie X, Chen C, Shu J, Thapa RK, Nguyen V, Bian S, Kohalmi SE, Marsolais F, Zou J, Cui Y 2021. LEAFY COTYLEDON1 expression in the endosperm enables embryo maturation in Arabidopsis. Nature Communications 12, 3963 (open access).

Feyissa BA, Amyot L, V, Papadopoulos Y, Kohalmi SE, Hannoufa A 2021. Involvement of the miR156/SPL module in flooding response in Medicago sativa. Scientific Reports 11, 3243 (open access).

Shu J, Chen C, Li C, Thapa RK, Song J, Xie X, Nguyen V, Bian S, Liu J., Kohalmi SE, Cui Y 2020. Genome-wide occupancy of Arabidopsis SWI/SNF chromatin remodeler SPLAYED provides insights into its interplay with its close homolog BRAHMA and Polycomb proteins. Plant Journal 106, 200-213.

Feyissa BA, Renaud J, Nasrollahi V, Kohalmi SE, Hannoufa A 2020. Transcriptome-IPMS analysis reveals a tissue-dependent miR156/SPL13 regulatory mechanism in Alfalfa drought tolerance. BMC Genomics. 21, 721.

Shu J, Chen C, Kohalmi SE, Cui Y 2020. Evidence that AGL17 is a significant downstream target of CLF in floral transition control. Plant Signaling and Behaviour 15 17666851

Feyissa BA, Arshad M, Gruber M, Kohalmi SE, Hannoufa A. The interplay between miR156-SPL13 and DFRWD40-1 in regulating drought tolerance in alfalfa, BMC Plant Biology 19, 434.

Chen C, Shu J, Li C, Thapa RK, Nguyen V, Yu K, Yuan Z-C, Kohalmi SE; Liu J, Marsolais F, Huang S, Cui Y. 2019. RNA polymerase II independent recruitment of SPT6L at transcription start sites in Arabidopsis. Nucleic Acid Research 47, 6714-6725.

Shu J, Chen C, Thapa R, Bian S, Nguyen V, Yu K, Yuan Z-C, Liu J, Kohalmi SE, Li C, Cui Y Genome-wide occupancy of H3K27 methyltransferases CURLY LEAF and SWINGER in Arabidopsis seedlings. Plant Direct e00100.

Saatian B, Austin RS, Tian G, Chen C, Nguyen V, Kohalmi SE, Geelen D, Cui Y Analysis of a novel mutant allele of GSL8 reveals its key roles in cytokinesis and symplastic trafficking in Arabidopsis. BMC Plant Biology 18 295.

Abolhassani Rad S, Clayton EJ, Cornelius EJ, Howes TR, Kohalmi SE Moonlighting proteins: Putting the spotlight on enzymes. Plant Signaling and Behavior 13, e1517075 (invited).

Kohalmi SE, Clayton EJ Tinker, Tailor, soldier, enzyme. Nature Plants 3, 848-849 (invited).

Chen C, Li C, Wang Y, Renaud J, Tian G, Kambhampati S, Saatian B, Nguyen V, Hannoufa A, Marsolais, Yuan Z-C, Yu K, Austin R, Liu J, Kohalmi SE, Wu K, Huang S, Cui Y 2017. Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Nature Plants, 3, 814–824.

Dezfulian MH, Foreman C, Jalili E, Pal M , Dhaliwal RK, Roberto DKA, Imre KM, Kohalmi SE, Last LL, Crosby WL 2017. Acetolactate synthase regulatory subunits play divergent and overlapping roles in Arabidopsis BMC Plant Biology 17, 71.

Bross CD, Howes TR, Abolhassani Rad S, Kljakic, O, Kohalmi SE Subcellular locations of Arabidopsisarogenate dehydratases suggests novel and differential roles.  Journal of Experimental Botany 68, 1425-1440.      

Wang Z, Wang Y, Kohalmi SE, Amyot L and Hannoufa A 2016. SQUAMOSA PROMOTER BINDING PROTEIN LIKE 2 controls floral organ development and plant fertility by directly activating ASYMMETRIC LEAVES 2 in Arabidopsis thaliana. Plant Molecular Biology, 92 661-674.

Saberianfar R, Sattaradeh A, Joensuu JJ, Kohalmi SE, Hanson M, Menassa R 2016. Protein bodies in leaves exchange contents through the endoplasmic reticulum. Frontiers in Plant Sciences. 7, art no. 893.

Li C, Gu L, Lei Gao L, Chen C, Wei C-Q, Qiu Q, Chien C-W, Wang S, Jiang L, Ai L-F, Chen C-Y, Yang S, Nguyen V, Qi Y, Snyder MP, Burlingame AL, Kohalmi SE, Huang S, Cao X, Wang Z-Y, Wu K, Chen X, Cui Y Concerted actions of histone H3 lysine 27 demethylase REF6 and chromatin-remodeling ATPase BRM in Arabidopsis. Nature Genetics 48, 687-693.

Li C, Chen C, Gao L, Yang S, Nguyen V, Shi X, Siminovitch K, Kohalmi SE, Huang S, Wu K, Chen X, Cui Y 2015. The Arabidopsis SWI2/SNF2 chromatin remodeler BRAHMA regulates polycomb function during vegetative development and directly activates the flowering repressor gene SVP. PLoS Genetics 11, doi: 10.1371.

Feeney M, Frigerio L, Kohalmi SE, Cui Y, Menassa R 2013 Reprogramming cells to study vacuolar development. Frontiers in Plant Sciences 4, 1-9.

Gutierrez SP, Saberianfar R, Kohalmi SE, Menassa R 2013. Protein body formation in stable transgenic tobacco expressing elastin-like polypeptide and hydrophobin fusion proteins. BMC Biotechnology, 13, art no 40.

Kaldis A, Ahmad A, Reid A, McGarvey B, Brandle J, Ma S, Jevnikar A, Kohalmi SE, Menassa R 2013. High level production of human interleukin-10 fusions in tobacco suspension cultures. Plant Biotechnology Journal 11, 535-545.

Wang X, Kohalmi SE, Svircev A, Wang A, Sanfaçon H, Tian L 2013. Silencing of host factor eIF(iso)4E gene confers Plum Pox Virus resistance in plum. PLoS ONE 8, art no e50627.

Tian G, Lu Q, Kohalmi SE, Rothstein SJ, Cui Y 2012. Evidence that Arabidopsis Ubiquitin C terminal Hydrolases 1 and 2 associate with the 26S proteasome and the TREX-2 complex. Plant Signaling and Behaviour, 7.

Garrett JJ, Meents MJ, Blackshaw MT, Blackshaw LC, Hou H, Alexander V, Erickson JL, Styranko DM, Kohalmi SE, Schultz EA 2012. A novel semi-dominant allele of MONOPTEROS indicates that negative regulation of auxin response is required for auxin canalization during vein formation. Planta 236, 297-312.

Tian, G ., Lu, Q., Zhang, L., Kohalmi, S.E., Cui, Y. 2011.  Detection of protein interactions in plant using a gateway compatible bimolecular fluorescence complementation (BiFC) system. Journal of Visualized Experiments doi: 10.3791/3473.

Bross, C.D., Corea, O.R.A., Kaldis, A., Menassa, R., Bernards, M.A., Kohalmi, S.E. 2011. Complementation of the pha2 yeast mutant suggests functional differences for arogenate dehydratases from Arabidopsis thaliana. Plant Physiology and Biochemistry, 49, 882-890.

Brandsma M., Diao H., Wang X., Kohalmi S.E., Jevnikar A.M., Ma S. 2010. Plant-derived recombinant human serum transferrin demonstrates multiple functions. Plant Biotechnology Journal, 8, 489-505.

Lu Q., Tang X., Tian G., Wang F., Liu K., Nguyen V., Kohalmi S.E., Keller W.A., Tsang E.W., Harada J.J., Rothstein S.J., Cui Y. (2010) Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Plant Journal, 61, 259-270.

Brandsma M., Wang X., Diao H., Kohalmi S.E., Jevnikar A.M., Ma S. 2009. A proficient approach to the production of therapeutic glucagon-like-1 (GLP-1) peptide in transgenic plants. The Open Biotechnology Journal. 3, 57-66

Cho, M.-H., Corea, O.R.A., Laskar, D.D., Anterola, A.M., Moog-Anterola, F.A., Hood, R.L., Kohalmi, S.E., Bernards, M.A., Kang, C, Davin, L.B., Lewis N.G. 2007. Phenylalanine biosynthesis in Arabidopsis thaliana: Identification and characterization of arogenate dehydratases. Journal of Biological Chemistry 282, 30827-30835.

Gudynaite-Savitch, L., Loiselay, C., Savitch, L.V., Simmonds, J., Kohalmi, S.E., Choquet, Y. Hüner, N.P.A. 2007. The small domain of cytochrome f from the psychrophilic Chlamydomonas raudensis UWO 241 modulates the apparent molecular mass and decreases the accumulation of cytochrome f in the mesophile Chlamydomonas reinhardtii. Biochemistry and Cell Biology 85, 616-627.

Gudynaite-Savitch, L., Gretes, M., Morgan-Kiss, R.M., Savitch, L.V., Simmonds, J., Kohalmi, S.E., Hüner, N.P.A. 2006. Cytochrome f from the Antarctic psychrophile, Chlamydomonas raudensis UWO241: structure, sequence, and complementation in the mesophile, Chlamydomonas reinhardtii. Molecular Genetics and Genomics, 275, 387-398.

Wakem M.P., and Kohalmi S.E. 2003. Mutation in the ap2-6 allele causes recognition of a cryptic splice site. Journal of Experimental Botany 54, 2655-2660.

Bellaoui M., Pidkowich M.S., Samach A., Kushalappa K., Kohalmi S.E., Crosby W.L. and Haughn G.W. 2001.The Arabidopsis BELL1 and KNOX TALE homeodomain proteins interact through a domain conserved between plants and animals. Plant Cell 13,3455-2470.

Pelaz S., Gustafson-Brown C., Kohalmi S.E., Crosby W.L., and Yanofsky M.F. 2001. APETALA1 and SEPALLATA3 interact to promote flower development. Plant Journal 26, 385-394.