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Western Chemistry Mass Spectrometry Facililty

Also check out the site of the UWO Biological Mass Spectrometry Laboratory for additional mass spec instrumentation at Western. 

High Resolution Mass Spectrometry

Thermo Scientific DFS (Double Focusing Sector) mass spectrometer for HRMS

A Thermo Scientific DFS (Double Focusing Sector) mass spectrometer, utilizing a reversed Nier Johnson geometry, is the workhorse instrument used for high resolution mass spectrometry and structure confirmation of organic and inorganic species. A resolution of > 60000 (at 10% valley) can be achieved on the instrument. The DFS has a mass range of 0-1200 m/z at full acceleration and up to 6000 m/z at reduced acceleration. 

Samples can be analyzed using two modes of ionization:

and samples can be introduced in a variety of ways:

This instrument is run as a sample service in the Chemistry Department; primarily for internal use but also for other research groups at UWO. It is also available to industry and government agencies at a reasonable cost.
For more information about fees and availability contact Doug Hairsine at 519-661-2111 ext. 86323 or scidwh@uwo.ca.

Electrospray Time of Flight Mass Spectrometry (ESI MS)

The Bruker microOTOF 11 is used for the analysis of organometallic samples as well as protein samples, having a mass range 60 -20,000 m/z. Samples are dissolved in a solution and infused to the electro sprayer by a syringe pump, where they are ionized (ESI) for mass spec analysis.

The department also has a Micromass LCT mass spectrometer. The purchase of this instrument became possible through a CRC-CFI grant to Dick Puddephatt. The instrument has a mass range of (m/z) 10,000 at a resolution of 5,000.

Another CRC-CFI grant to Lars Konermann allowed the acquisition of a Micromass/Waters Q-TOF Ultima LC-MS/MS system. This instrument can operate in "V" and in "W" reflectron mode and is capable of producing mass spectra at a resolution of up to 20,000 (FWHM).

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Bruker Daltonics Reflex IV

Bruker Daltonics Reflex IV MALDI-TOF

Our Bruker Daltonics Reflex IV is a top-of-the-line research grade MALDI time-of-flight (TOF) instrument equipped with a linear/reflectron mass analyzer and post-source decay (PSD) capability. Its primary application is in proteomics, where it is used in combination with capillary electrophoresis. The extended mass range detector provides enhanced sensitivity up to m/z of 300,000 which is ideal for the nano-liter sample size of capillary electrophoresis. The resolution in reflectron mode is > 20,000. This instrument was funded through a CFI New Opportunities grant to Ken Yeung.

Routine GCMS

The Shimadzu GCMS-QP2010 is a bench top gas chromatograph/ quadrupole mass spectrometer for high precision gc/ms analysis. The instrument is ideal for qualitative sample analysis and identification of unknowns or for the quantitation of trace constituents. The Shimadzu GCMS-QP2010 is an electron impact (EI) only instrument and has a mass range 10 -1000 m/z, and uses a Shimadzu AOC-20i auto injector for automated sample injecting.  Similar instruments exist in individual research labs.