Ph.D. (University of Illinois at Urbana-Champaign), Postdoctoral Fellow Yale University
Canada Research Chair (Tier 2) in Chemical Biology
National Science Foundation Postdoctoral Fellowship in Biological Informatics
PhRMA Foundation Postdoctoral Fellowship in Informatics
Institute for Genomic Biology Research Fellowship, University of Illinois
The O’Donoghue lab investigates how proteome diversity generated by either protein modification or mistranslation impacts pathways to cancer and neurodegeneration. Proteins involved in major human diseases undergo significant posttranslational modification. Protein modifications relay chemical signals to alter cell fate and gene expression. Certain signals lead to disease while others do not. My lab established a leadership role in the area of genetic code expansion to investigate the role of disease-linked protein modifications. My lab engineered new methods to produce proteins with programmed modifications and elucidated their role in signaling pathways linked to disease. Recent breakthroughs in my lab enable programmed protein modification in living human cells. We recently developed novel optogenetic tools to visualize mistranslation in live cells. Expanding beyond individual proteins, we are now leading efforts to reveal the ability of naturally occurring tRNA mutants to induce proteome-wide mistranslation in models of neurodegenerative disease.
Biochemistry 4415b/Chemistry 4415b - Applications of Synthetic Biology and Chemical Genetics in Medicine
Biochemistry 9703/Chemistry 9703 - Chemical Biology
Selected Recent Publications
N. Balasuriya*, M. Kunkel*, X. Liu, K. Biggar, S. Li, A. Newton†, & P. O’Donoghue† (2018) Genetic code expansion and live cell imaging reveal that Thr308 phosphorylation is irreplaceable and sufficient for Akt1 activity. The Journal of Biological Chemistry. doi: 10.1074/jbc.RA118.002357.
D. E. Wright, Z. Altaany, Y. Bi, Z. Aplerstein, & P. O’Donoghue†. (2017) Acetylation regulates thioredoxin reductase oligomerization and activity. Antioxidants & Redox Signaling. doi: 10.1089/ars.2017.7082.
P. O’Donoghue†, & I. U. Heinemann†. (2017) Biochemistry of synthetic biology – recent developments. Biochim Biophys Acta. 1861(11PB): 2945-2947.
J. T. Lant, M. D. Berg, D.H.W Sze, K. S. Hoffman, I. C. Akinpelu, T. M. Turk, I. U. Heinemann, M. L. Duennwald, C. J. Brandl, & P. O’Donoghue†. (2017) Visualizing tRNA-dependent mistranslation in human cells. RNA Biology. doi: 10.1080/15476286.2017.1379645.
M. D. Berg†, K. S. Hoffman, J. Genereaux, S. Mian, R. S. Trussler, D. B. Haniford, P. O’Donoghue & C. Brandl†. (2017) Evolving mistranslating tRNAs through a phenotypically ambivalent intermediate in Saccharomyces cerevisiae. Genetics. 206:1865-18792. (IF = 6.0). Featured in Genetics Society of America E-news (8/16/17); B. Starr ‘New & Noteworthy’ article feature at Saccharomyces Genome Database: https://www.yeastgenome.org/blog/that-sweet-spot-of-mistranslation (3/8/17).