Peter Chidiac
Associate Professor
 |
PH.D. University of Toronto |
Cells within an organism communicate with other cells by releasing chemical messengers, such as hormones and neurotransmitters. These bind to and activate proteins called receptors, causing changes in cell behaviour. The majority of receptors work by promoting the binding of GTP to intracellular G proteins. Thus activated, G proteins are able to regulate effector proteins to modulate second messenger production, which in turn causes further biochemical changes within the cell. G proteins become deactivated when the GTP bound to them is hydrolyzed to GDP. The rate of GTP hydrolysis is accelerated by RGS (regulator of G protein signaling) proteins. Thus, receptors and RGS proteins respectively activate and deactivate G proteins. Whereas the function and therapeutic importance of receptors have been studied for nearly a century, however, RGS proteins were unknown until relatively recently, and their physiological role for the most part remains to be established. The research in my laboratory focusses on how the activities of G proteins, receptors, effectors, and RGS proteins are regulated, and how these signaling proteins interact with one another. Signaling is assessed via GTPase activity, second messenger production and biochemical changes further downstream. Additionally we study protein-protein binding, and monitor changes in protein expression and intracellular localization. Many human diseases are characterized by deleterious G protein-mediated signals and/or treatable by drugs that are targeted to G protein pathways. In collaboration with other members of the University of Western Ontario Heart Failure Research Group, we are examining the ways in which signaling through G proteins contributes to the development of cardiac hypertrophy and heart failure, and examining how the selective attenuation of these signals might slow down the progression of this disease.
Abramow-Newerly M, Ming H, Chidiac P. Modulation of subfamily B/R4 RGS protein function by 14-3-3 proteins. Cell Signal. 2006 May 23; [Epub ahead of print] Nunn C, Mao H, Chidiac P, Albert PR. RGS17/RGSZ2 and the RZ/A family of regulators of G-protein signaling. Semin Cell Dev Biol. 2006 Jun;17(3):390-9. Epub 2006 Apr 7. Zou MX, Roy AA, Zhao Q, Kirshenbaum LA, Karmazyn M, Chidiac P. RGS2 is upregulated by and attenuates the hypertrophic effect of alpha(1)-adrenergic activation in cultured ventricular myocytes. Cell Signal. 2006 Oct;18(10):1655-63. Epub 2006 Mar 6. Abramow-Newerly M, Roy AA, Nunn C, Chidiac P. RGS proteins have a signalling complex: interactions between RGS proteins and GPCRs, effectors, and auxiliary proteins. Cell Signal. 2006 May;18(5):579-91. Epub 2005 Oct 13. Review. Roy AA, Baragli A, Bernstein LS, Hepler JR, Hebert TE, Chidiac P. RGS2 interacts with Gs and adenylyl cyclase in living cells. Cell Signal. 2006 Mar;18(3):336-48. Epub 2005 Aug 10.
