Alemayehu M, Dragan M, Pape C, Siddiqui I, Sacks DB, Di Guglielmo GM, Babwah AV, Bhattacharya M. β-Arrestin2 regulates lysophosphatidic acid-induced human breast tumor cell migration and invasion via Rap1 and IQGAP1.PLoS One. 2013;8(2):e56174. doi: 10.1371/journal.pone.0056174. Epub 2013 Feb 6.
Babwah AV, Pampillo M, Min L, Kaiser UB, Bhattacharya M. Single-Cell Analyses Reveal That KISS1R-Expressing Cells Undergo Sustained Kisspeptin-Induced Signaling That Is Dependent upon An Influx of Extracellular Ca2+ Endocrinology. 2012 Oct 15. [Epub ahead of print]
Zajac M, Law J, Cvetkovic D, Pampillo M, McColl L, Pape C, Postovit L, Di Gugliemo J, Babwah AV, Bhattacharya M. GPR54 transactivates EGFR to promote breast cancer cell invasiveness. PLoS One (2011) PLoS One. 2011;6(6):e21599.
Szereszewski JM, Pampillo M, Offermanns S, Bhattacharya M, Babwah AV. GPR54 regulates ERK1/2 activity and hypothalamic gene expression in a Gαq/11 and β-arrestin-dependent manner. PLoS One. (2010) 5: e12964.
Re M, Pampillo M, Savard M, McArdle CA, Millar RP, Conn M, Gobeil F Jr, Bhattacharya M, Babwah AV. The human gonadotropin releasing hormone type I receptor is a functional intracellular GPCR expressed on the nuclear membrane. PLOS One (2010) 5: e11489.
Li T, Alemayehu M, Aziziyeh IA, Pape MC, Pampillo M, Mills GB, Babwah AV, Bhattacharya M. b-arrestins and RalGTPases regulate lysophosphatidic acid mediated breast cancer cell migration and invasion. Molecular Cancer Research (2009) 7: 1064-77 (Cover feature)
Pampillo M, Camuso N, Taylor JE, Szereszewski JM, Ahow M, Zajac M, Millar RP, Bhattacharya M, Babwah AV. Molecular regulation of GPR54 activity by GRK-2 and β-arrestins. Molecular Endocrinology (2009) 13:2060-2074
Aziziyeh IA, Alemayehu M, Li T, Pape MC, Pampillo M, Possmayer F, Babwah AV, Bhattacharya M. Dual regulation of lysophophatidic acid receptor signaling by RalGTPases and GRK2. Cellular Signalling. (2009) 21:1207-17
Cavanagh PC, Dunk C, Pampillo M, Kahiri C, Han V, Lye S, Bhattacharya M, Babwah AV. GnRH-Regulated Chemokine Expression in the Human Placenta. American Journal of Physiology: Cell Physiology (2009) 297:C17-27
In Canadian women, breast cancer is the most frequently diagnosed cancer, accounting for an estimated 30% of all cancer cases. The high mortality rate from breast cancer is largely due to the metastatic spread of the disease and therefore suppression of metastasis is an important issue in the treatment of the disease. However, the underlying mechanism(s) regulating metastasis are largely unknown. We are interested in how the signaling and trafficking of G protein-coupled receptors regulates breast cancer metastasis, using various molecular and biochemical techniques to study gene expression and protein-protein interactions in cancer cells. We also use fluorescent reporter molecules to study receptor-mediated intracellular signalling events in real-time, in single live cells and visualized by laser scanning confocal microscopy. The research program is comprised of the following projects:
