VWR Biobar at UWO

Fred Williams DSB 3014 biobar@uwo.ca

EZ-Vision® Dye-as-Loading Buffer, 6X, AMRESC

Easy and Convenient
Instant Results
Eliminates Hazardous Ethidium Bromide Use

Provides a safe, non-mutagenic alternative to ethidium bromide for instantaneous fluorescent DNA band visualization. This environmentally friendly dye requires no special handling or disposal, and will not incur hazardous shipping, storage, or waste charges. Supplied in a 6X loading buffer that is added to the DNA sample and can be run on a gel and visualized immediately on a UV transilluminator. Requires no post electrophoresis staining or destaining and has a very broad emission spectra with a peak near 450nm that works with most existing filters for gel documentation. Three different tracking dye options are available. Each version contains the same non-hazardous fluorescent DNA dye and loading buffer. EZ-Vision® One is one tracking dye migrating at approximately 10bp in a 1% agarose gel. EZ-Vision® Two is two tracking dyes migrating at approximately 4,000bp and 400bp in a 1% agarose gel. EZ-Vision® Three is three tracking dyes migrating at approximately 4,000bp, 400bp, and 10bp in a 1% agarose gel.

qScript™ cDNA SuperMix, Quanta Biosciences

SuperMix provides a sensitive and easy-to-use solution for two-step qRT-PCR. This 5x concentrated master mix provides all necessary components (except RNA template) for first-strand synthesis including: buffer, dNTPs, MgCl2, primers, RNase inhibitor protein, qScript reverse transcriptase, and stabilizers. qScript™i s a RNase H(+) derivative of MMLV-RT, optimized for reliable cDNA synthesis over a wide dynamic range of input RNA. The unique blend of oligo (dT) and random primers in the qScript™ cDNA SuperMix works exceptionally well with a wide variety of targets. This blend is optimized for the production of targets \<1kb in length. qScript™ cDNA SuperMix produces excellent results in both real-time and conventional RT-PCR.

Fetal Bovine Sera, PAA Laboratories

Animal sera are used as the main source for growth factors, hormones, and other cell-stimulating components. Blood is collected from healthy animals, centrifuged, and tested for endotoxin and hemoglobin levels before processing and further testing. Fetal bovine serum has been tested for viruses and microorganisms. It supports most cell lines, acts as a growth-promoting additive, and acts as a stabilizer in diagnostic assays. It can also be used to surface coat culture vessels and inactivate trypsin. Fetal bovine serum GOLD is completely defined, but is not a synthetic product, and contains no additives from other sera. GOLD serum is chromatographically purified and fractionated, then the individual constituents are recombined in a defined composition, eliminating batch-to-batch variation. It is also distinguished by a very low content of IgG antibodies. Organic fetal bovine serum is collected from antibiotic-free animals that have been fed organic greens. ES cell tested serum supports the growth of undifferentiated ES cells and has low nonspecific stimulation. It is ideal for the morphology of stem cell colonies and for elevated expression of the stem cell marker phosphatase. Pharma grade fetal bovine serum receives the most intensive testing according to the latest FDA and Ph. Eur. guidelines. Pharma grade serum offers the highest levels of safety, especially regarding possible virus contamination. Heat inactivated serum has been heated to inactivate the complement system, antibodies, and other active enzymes. Dialyzed serum has a reduced content of hormones and molecules with a molecular weight of \<10kDa, such as nucleotides and amino acids. Charcoal stripped fetal bovine serum has a strongly reduced hormone concentration with minimized batch-to-batch variation. The IgG content of IgG stripped fetal bovine serum has been reduced by HCI chromatography to \<5µg/mL, ensuring unimpaired growth promotion. IgG stripped serum is ideal for hybridoma cell culture and the production of monoclonal antibodies. USDA approved serum is manufactured from raw materials sourced from Central American countries such as Mexico and Panama. “Various” origin indicates sera that has been manufactured from raw materials sourced from Australia, Canada, USA, or Central American countries. All sera products are sterile, filtered, and packaged in unbreakable, gamma-irradiated PET bottles. Production facilities comply with cGMP guidelines and are registered with the FDA and EDQM. Fetal bovine serum receives additional testing according to 9CFR and EP regulations. Products are fully traceable and records are available upon request.

Agarose I™

All-Purpose, High-Purity Agarose Exceptional Band Resolution and Clarity Nuclease- and Protease-free A standard melting/gelling agarose, suitable for routine nucleic acid analytical/preparative applications. It has a low EEO for shorter electrophoretic runs without compromising resolution. Excellent for blotting techniques and general manipulations.

Fluorescent Next Gel® Polyacrylamide Electrophoresis

Ready-to-pour SDS polyacrylamide solutions—just add APS
and TEMED
Faster gel casting with no stacking gel required
Convenient fluorescent dye stains proteins during electrophoresis and requires no destaining post-electrophoresis
Broad range of separation
High resolution of protein bands
Stable >1 year at room temperature

AMRESCO’s NEXT GEL® products, which are proprietary, ready-to- pour blends of acrylamide, bis-acrylamide, gel buffer, and SDS, are available with a convenient fluorescent stain as Fluorescent NEXT GEL® and Fluorescent SPRINT NEXT GEL®. The fluorescent stain enables rapid visualization following electrophoretic separation of denatured proteins without the need for post-run staining or destaining. The gels are simply placed on a UV transilluminator at 312 nm, causing the fluorescent probe to covalently cross-link to the protein. Within 3–5 minutes the protein bands appear.
The Fluorescent NEXT GEL® products have been formulated to reliably separate proteins and are fully compatible with standard 1D and 2D SDS-PAGE and downstream applications. Fluorescent NEXT GEL®products are available in multiple acrylamide percentages and are accompanied by sufficient running buffer. NEXT GEL® Sample Loading Buffer, 4X and NEXT GEL® Transfer Buffer, 10X are available separately and are recommended for optimum gel and transfer performance. NEXT GEL® products are also fully compatible with AMRESCO’s Rapid Transfer Buffer, 10X and Rapid Western Blotting Kits.
Includes: NEXT GEL® Running Buffer, 20X

NEXT GEL® Polyacrylamide Gel Electrophoresis Solutions

Ready-to-Pour SDS Polyacrylamide Solutions
Faster Electrophoresis Times using SPRINT NEXT GEL® Formulations
Broad Range of Separation—3.5 kDa and 212 kDa on the Same Gel
Stable >1 Year at Room Temperature

NEXT GEL® is a ready-to-pour premixed solution of acrylamide, bisacrylamide, gel buffer, and SDS that enables superior, ultra-fine resolution of proten bands. The unique acrylamide matrix slows the migration of proteins, eliminating the need for a stacking gel. This not only saves time, but also permits the proteins to run across a longer gel surface resulting in increased resolution. The proprietary chemistry has gradient-like properties, which enables the separation of small peptides and high molecular weight proteins in the same gel.
NEXT GEL® and SPRINT NEXT GEL® are ideal for mini-gel electrophoresis, while Turbo NEXT GEL® is optimized for 16x16cm gels. SPRINT NEXT GEL® and Turbo NEXT GEL® can be run at high voltage without overheating, resulting in reduced electrophoresis time.

Ordering Information: All solutions include 20X running buffer. Sample kit contains 30mL of each solution (5%, 7.5%, 10%, 12.5%, and 15%), as well as 20X running buffer and 4X sample loading buffer.

Visiglo™ And Visiglo Plus™ Chemiluminescent Substrate Kits

Highly sensitive substrates save precious protein samples and antibodies
Low background without compromising signal intensity

VisiGlo™ HRP and VisiGlo Plus™ HRP are luminol-based chemiluminescent substrates designed for the rapid and sensitive detection of peroxidase-labeled conjugates. They offer increased sensitivity compared to chromogenic substrates in both blotting and ELISA applications. VisiGlo™ HRP and VisiGlo Plus™ HRP can be detected using x-ray film and chemiluminescent imagers in blotting applications or using a luminometer for ELISA. VisiGlo Plus™ HRP is AMRESCO’s most sensitive chemiluminescent substrate and is ideal for detecting proteins that are in low abundance.

VisiGlo™ AP and VisiGlo Plus™ AP are the chemiluminescent substrates of choice for easy, reliable detection of alkaline-phosphatase in Western blotting or ELISA. Both products are single component, ready-to-use substrates that do not require special blocking solutions. These substrates glow up to 5 days, allowing for repeated exposure or luminosity reading.

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March 14, 2012