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Welcome to the Kang Lab
Dr. C. Yong Kang (Dipl. Vet. Sci Denmark ; BSc Korea ; PhD McMaster; F.R.S.C.
Office: Rm 129 Siebens-Drake Research Institute
Phone: 519 661-3226
Fax: 519 661-3499
The progress in understanding the molecular biology of human immunodeficiency virus has been enormous. However, all this progress has yet to crack the central mystery of AIDS and an effective vaccine to prevent AIDS has yet to be developed. The increasing use of genetic engineering and other modern technology have allowed researchers to identify the regions of HIV proteins which stimulate the immune response. Our research is involved in the production of virus-like particles known as pseudovirions, using an unprocessed internal protein of HIV which will carry a crucial portion of the HIV envelope protein to produce protective immune responses. We have already succeeded in making pseudovirions in insect cells using recombinant baculoviruses as the carrier of a portion of HIV genes. Insect cells in culture infected with recombinant baculovirus produce large quantities of non-infectious pseudovirions. These pseudovirions, carrying many important regions of both internal proteins and external envelope proteins of AIDS virus, will be used to generate the neutralizing antibodies which will prevent the virus infection and also generate cytotoxic T lymphocytes, which are designed to kill and destroy the AIDS virus-infected cells in the body. In addition to the development of subunit AIDS vaccines, we are also studying the function of different regulatory proteins of HIV in order to understand the strategy of replication. Our laboratory is also involved in research to understand the genetics and evolution of segmented RNA viruses and to understand the strategy of replication of RNA viruses with a segmented genome. To achieve this goal, we have carried out the cloning, sequencing and expression of prototype strains of hantaviruses, analyzed the viral-specific glycoprotein biosynthesis and maturation, and characterized the viral-specific mRNA species. We are carrying out experiments to reach our ultimate goal by characterizing viral-specific mRNAs and other subgenomic RNAs from virus-infected cells, study the post transcriptional modification of viral-specific mRNAs, analyze the viral genomic RNA replication, determine the genomic reassortment between the different serotypes of hantaviruses, and investigate the possibility of genetic recombination between the different serotypes of hantaviruses. Better understanding of hantavirus genetics and the strategy of virus replication may lead us to understand the genetic stability and evolution of segmented RNA viruses and to understand the strategy of replication of viruses with segmented genomes.
Li Y, Bergeron JJ, Luo L, Ou WJ, Thomas DY, Kang CY . Effects of inefficient cleavage of the signal sequence of HIV-1 gp 120 on its association with calnexin, folding, and intracellular transport. Proc Natl Acad Sci USA 93(18):9606-9611, 1996 .
Luo L, Li Y, Chang JS, Cho SY, Kim TY, Choi MJ, Cheong HS, Kim HJ, Ahn HJ, Min MK, Chun BH, Jung SM,
Kang CY , Luo L, Wainberg MA, Li Y. Development of HIV/AIDS vaccine using chimeric gag-env virus-like particles. Biol Chem 380:353-364, 1999 .
Li, Y., Luo, L., Thomas, D.Y., Kang, CY . The HIV-1 Env protein signal sequence retards its cleavage and down regulates the glycoprotein folding. Virology 272: 417-428, 2000 .
Kim, G.N., Choi, W.Y., Park, M., and Kang, CY . Replicated transcriptions of viral RNAs by recombinant L proteins of New Jersey serotype of vesicular stomatitis virus. Virus Research, 90: 347-364, 2002 .
Luo, L., Li, Y., and Kang, CY . Budding and secretion of HIV Gag-Env virus-like particles, from recombinant human adenovirus infected cells. Virus Research 92: 75-82, 2003 .