Also check out the site of the UWO Biological Mass Spectrometry Laboratory for additional mass spec instrumentation at Western.
A Thermo Scientific DFS (Double Focusing Sector) mass spectrometer, utilizing a reversed Nier Johnson geometry, is the workhorse instrument used for high resolution mass spectrometry and structure confirmation of organic and inorganic species. A resolution of > 60000 (at 10% valley) can be achieved on the instrument. The DFS has a mass range of 0-1200 m/z at full acceleration and up to 6000 m/z at reduced acceleration.
Samples can be analyzed using two modes of ionization:
and samples can be introduced in a variety of ways:
This instrument is run as a sample service in the Chemistry Department; primarily for internal use but also for other research groups at UWO. It is also available to industry and government agencies at a reasonable cost.
For more information about fees and availability contact Doug Hairsine at 519-661-2111 ext. 86323 or firstname.lastname@example.org.
The Bruker microOTOF 11 is used for the analysis of organometallic samples as well as protein samples, having a mass range 60 -20,000 m/z. Samples are dissolved in a solution and infused to the electro sprayer by a syringe pump, where they are ionized (ESI) for mass spec analysis.
The department also has a Micromass LCT mass spectrometer. The purchase of this instrument became possible through a CRC-CFI grant to Dick Puddephatt. The instrument has a mass range of (m/z) 10,000 at a resolution of 5,000.
Another CRC-CFI grant to Lars Konermann allowed the acquisition of a Micromass/Waters Q-TOF Ultima LC-MS/MS system. This instrument can operate in "V" and in "W" reflectron mode and is capable of producing mass spectra at a resolution of up to 20,000 (FWHM).
Our Bruker Daltonics Reflex IV is a top-of-the-line research grade MALDI time-of-flight (TOF) instrument equipped with a linear/reflectron mass analyzer and post-source decay (PSD) capability. Its primary application is in proteomics, where it is used in combination with capillary electrophoresis. The extended mass range detector provides enhanced sensitivity up to m/z of 300,000 which is ideal for the nano-liter sample size of capillary electrophoresis. The resolution in reflectron mode is > 20,000. This instrument was funded through a CFI New Opportunities grant to Ken Yeung.
The Shimadzu GCMS-QP2010 is a bench top gas chromatograph/ quadrupole mass spectrometer for high precision gc/ms analysis. The instrument is ideal for qualitative sample analysis and identification of unknowns or for the quantitation of trace constituents. The Shimadzu GCMS-QP2010 is an electron impact (EI) only instrument and has a mass range 10 -1000 m/z, and uses a Shimadzu AOC-20i auto injector for automated sample injecting. Similar instruments exist in individual research labs.