Ken K.-C. YeungKen K.-C. Yeung
Associate Professor
B.Sc., Ph.D., Calgary
NSERC PDF, Stanford and Alberta
Office: Med Sci Bldg 374, Lab 373
(519) 661-2111 ext 86439

Research Group Homepage

Analytical and Bioanalytical Chemistry & Separations and Mass Spectrometry

Current Research Programs:

Even the latest analytical technologies are limiting in detecting proteins at trace level (below femto-moles). As a result, a significant portion of the proteins in our bodies have not been identified, including many that could be very important targets for diagnostic, prognostic and therapeutic studies. In this group, the main research goal is to develop novel analytical methodologies which will enable the analysis for proteins at ultra trace level.

Recent developments in this group focused on the use of capillary electrophoresis (CE) to perform a number of sample preparation reactions at sub-microliter volumes. They include selective extraction, enrichment, disulfide reduction, proteolysis and separation of proteins. Current interest is on the integration of these reactions using magnetic microbeads for analyte transport at minuscule quantities.

Additional to the methodology development, applications in studying disease-related protein phosphorylation are also underway. Phosphorylation is one of the most important post translational modifications involved in many cellular regulatory mechanisms and transduction pathways. Uncontrolled phosphorylation has been linked to the development of many diseases including cancer. A long term research goal is to utilize the new methodologies to advance phospho-protein research in life sciences.

Selected Publications:

“Removal of sample background buffering ions and myoglobin enrichment via a pH junction created by discontinuous buffers in capillary electrophoresis” Christina J Booker, Samuel Sun, Sarah Woolsey, Jose S Mejia, Ken K.-C. Yeung. Journal of Chromatography A (2011) 5705-5711.

“In-capillary enrichment, proteolysis and separation using capillary electrophoresis with discontinuous buffers: application on proteins with moderately acidic and basic isoelectric points”, Chandra A. Nesbitt and Ken K.-C. Yeung. The Analyst (Royal Society of Chemistry, UK), Vol. 134, pages 65-71 (2009).

"Nanoliter-Volume Protein Enrichment, Tryptic Digestion, and Partial Separation based on Isoelectric Points for MALDI-MS”, Chandra A. Nesbitt, Kristina Jurcic and Ken K.-C. Yeung. Electrophoresis (2008) Vol. 29, pages 466-474.

“Selective Injection of Multiply Phosphorylated Peptides by Capillary Electrophoresis for Electrospray Ionization Mass Spectrometry Analysis”, Jennifer N.M. Ballard, Gilles A. Lajoie and Ken K.-C. Yeung.  Journal of Chromatography A (2007) Vol. 1156, pages 101-110.

“An Integrated Procedure of Selective Injection, Sample Stacking and Fractionation of Phospho-peptides for MALDI MS Analysis”, Haixia Zhang, Graeme K. Hunter, Harvey A. Goldberg, Gilles A. Lajoie and Ken K.-C. Yeung.  Analytica Chimica Acta (2007) Vol. 581, pages 268-280.

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