Jim StaplesWestern Science

Publications

2021 - present | 2011 - 2020 | 1995-2010

Publications 2021 - present

Coulson, S.Z., Guglielmo, C.G. and Staples, J.F. Flight muscle mitochondria are seasonally remodeled in a migratory songbird to increase oxidative capacity without increasing reactive oxygen species production. In Press. J. Exp. Biol.

Hutchinson, A.J., Duffy. B., Rego, L. and Staples J.F. 2024. Paper Towel Shredding: a novel, affordable, non-invasive method for detecting arousals in hibernating rodents. Lab Animal https://doi.org/10.1038/s41684-024-01362-x.

Lewis, C.T.A, Thomsen, E.J.M, Ognjanovic, M.M., Olsen, M,S., Laitila, J., Seaborne, R. A.E., Grønset, M.N., Zhang, C,. Iwamoto, H., Hessel, A.L., Merino, C., Amigó, N., Fröbert, O., Giroud, S., Sprenger, R.J., Staples, J.F., Goropashnaya, A., Fedorov, V., Drew, K.L., Ochala, J. 2024. Remodeling of skeletal muscle myosin metabolic states in small hibernating mammals. eLlife. https://doi.org/10.7554/eLife.94616.1

Duffy, B.E., Coulson, S.Z. and Staples, J.F. 2024. Mitochondrial techniques for physiologists. Comp. Biochem. Physiol. B. 271: https://doi.org/10.1016/j.cbpb.2024.110947

Hutchinson, A.J., Duffy. B. and Staples J.F. 2023. Electron transport system supercomplexes affect reactive oxygen species production and respiration in both a hibernator (Ictidomys tridecemlineatus) and a non-hibernator (Rattus norvegicus). 193:715–728 J. Comp. Physiol. B. https://doi.org/10.1007/s00360-023-01525-1

Duffy, B., Hayward, L. and Staples, J.F. 2023. Torpid 13-lined ground squirrel liver mitochondria resist anoxia-reoxygenation despite high levels of protein damage. J. Comp. Physiol. B. DOI: 10.1007/s00360-023-01515-3

Gerber, L., MacSween, C.E., Staples, J.F. and Gamperl, A.K. 2022. Cold-Induced Metabolic Depression in Cunner (Tautogolabrus adspersus): A Multifaceted Cellular Event. PLoS ONE 17(8):e0271086. doi: 10.1371/journal.pone.0271086

Staples, J.F., Mathers, K.E. and Duffy, B.E. 2022. Mitochondrial metabolism in hibernation: Regulation and Implications. Physiology, 37(5). doi: 10.1152/physiol.00006.2022

Lebenzon, J.E, Denezis, P., Mohammad, L., Mathers, K.E., Turnbull, K.F., Staples, J.F. and Sinclair B.J. 2022. Reversible mitophagy drives metabolic suppression in diapausing beetles. Proc. Natl. Acad. Sci. 119(30) . https://doi.org/10.1073/pnas.2201089119.*Featured in OUSTIDE JEB"*

Hutchinson, A.J.*, Duffy, B.M.* and Staples, J.F. 2022. Hibernation is super complex: distribution, dynamics, and stability of electron transport system supercomplexes in Ictidomys tridecemlineatus. Am. J. Physiol. R. 323(1):R28-42. https://journals.physiology.org/doi/abs/10.1152/ajpregu.00008.2022 *FEATURED IN "OUSTIDE JEB"*

Duffy, B.* and Staples, J.F. 2022. Arousal from torpor increases oxidative damage in the hibernating thirteen-lined ground squirrel Ictidomys tridecemlineatus.  Physiol. Biochem. Zool. 95(3):229-238. https://doi.org/10.1086/719931

Jensen, B*, Pardue, S., Duffy*, B., Kevil, C., Staples, J.F. and Fago, A. 2021. Suppression of mitochondrial respiration by hydrogen sulfide in hibernating 13-lined ground squirrels. Free Radical Biol. Med. 169:181-186. https://doi.org/10.1016/j.freeradbiomed.2021.04.009

MacCannell, A.D.V.* and Staples, J.F. 2021. Elevated ambient temperature accelerates aspects of torpor phenology in an obligate hibernator. J. Therm. Biol. 96: 102839. https://doi.org/10.1016/j.jtherbio.2021.102839

Publications 2011 - 2020

 Jariwala, K.A., Sherazi, A.A., Tazhitdinova, R., Shum, K., Guevorguian, P., Karagiannis, J., Staples, J.F. and Timoshenko, A.V. 2020. The association between increasing levels of O-GlcNAc and galectins in liver tissue of hibernating thirteen-lined ground squirrels (Ictidomys tridecemlineatus). Cell Tissue Res. 381(1):115-123. doi: 10.1007/s00441-020-03185-x

Toepfer, C., Garfinkel, A., Venturini, G., Wakimoto, H., Repetti, H., Alamo, L., Sharma, A., Agarwal, R., Ewoldt, J., Cloonan, P., Letendre, J., Olivotto, I., Colan, S., Ashley, E., Jacoby, D., Michelles, M., Redwood, C., Watkins, H., Day, S., Staples, J., Padron, R., Chopra, A., Ho, C., Chen, C., Pereira, A., Seidman, J. 2020. Myosin sequestration regulates sarcomere function, cardiomyocyte energetics, and metabolism, informing the pathogenesis of hypertrophic cardiomyopathy. Circulation. 141:828–842.

Wang, X., Staples, J.F., Watt, C.F., McKenzie and C.A. MacCannell, A.D.V. 2019. Thermoneutral temperature reduces liver volume but increases fat content in a mammalian hibernator. J. Therm. Biol. 83: 172-177: doi 10.1016/j.jtherbio.2019.05.015

Mathers, K.E.* and Staples, J.F. 2019. Differential post-translational modification of mitochondrial enzymes corresponds with metabolic suppression during hibernation. Am. J. Physiol. R. 317:R262-R269 doi.org/10.1152/ajpregu.00052.2019. Chosen as APSselect for "outstanding scientific discoveries".

MacCannell, A.D.V.*, Sinclair K.F., Tattersall, G., McKenzie, C.A., and Staples, J.F. 2019. Identification of a lipid-rich depot in the orbital cavity of the 13-lined ground squirrel. J. Exp. Biol. 222. doi:10.1242/jeb.195750. Featured in "Inside JEB"

MacCannell, A.D.V., Sinclair K.F., Staples, J.F. and McKenzie, C.A. 2019. Environmental temperature effects on adipose tissue growth in a hibernator. J. Exp. Biol. 222, doi: 10.1242/jeb.194548

MacCannell, A.D.V., Jackson, E.C., Mathers, K.E. and Staples, J.F. 2018. An improved method for detecting entrance and arousal in a mammalian hibernator using heart rate data. J. Exp. Biol. 221 doi: 10.1242/jeb.174508

Dugbartey, G.J., Hardenberg, M.C., Kok, W.F., Boerema, A.S., Carey, H.V., Staples, J.F., Henning, R.F. and Bouma, H.R. 2017. Renal mitochondrial response to low temperature in non-hibernating and hibernating species. Antioxidants and Redox Signalling. 27:599-617.

MacCannell, A., Sinclair, K., Friesen-Waldner, L. McKenzie, C. and Staples, J. 2017. Water-fat MRI reveals growth of brown adipose tissue without cold exposure in a hibernator. J. Comp. Physiol. 187:759-767 DOI: 10.1007/s00360-017-1075-8

McFarlane, S.V., Mathers, K.E. and Staples, J.F. 2017. Reversible temperature-dependent alterations of brown adipose tissue mitochondrial respiration during torpor in a mammalian hibernator. Am. J. Physiol. R. 312(3): R434-R442. DOI: 10.1152/ajpregu.00316.2016

Mathers, K.E., McFarlane, S.V., Zhao, L. and Staples, J.F. 2017. Regulation of mitochondrial metabolism during hibernation by reversible suppression of electron transport system enzymes. J. Comp. Physiol. B 187:227–234. DOI 10.1007/s00360-016-1022-0

Robertson, A., Schaltz, K., Neimanis, K., Staples, J.F. and McDonald*, A.E. 2016. Heterologous Expression of the Crassostrea gigas (Pacific Oyster) Alternative Oxidase in the Yeast Saccharomyces cerevisiae. J. Bioenerget. Biomembr. 48:509-520. DOI:10.1007/s10863-016-9685-5.

Staples J.F. 2016. Metabolic Flexibility: Hibernation, Torpor and Estivation. invited review Comprehensive Physiol. 6(2): 737-771.

Mathers, K.E. and Staples, J.F. 2015. Saponin-permeabilization is not a viable alternative to isolated mitochondria for assessing oxidative metabolism in hibernation. Biol. Open. 4:858-864. DOI - 10.1242/​bio.011544.

Dunlop, K., Cedrone, M., Staples, J.F and Regnault, T.R.H. 2015. Altered Fetal Skeletal Muscle Nutrient Metabolism Following an Adverse In Utero Environment and the Modulation of Later Life Insulin Sensitivity. Nutrients. 7: 1202-1216.

MacMillan H.A., Ferguson, L.V., Nicolai, A. Donini, A., Staples, J.F. and Sinclair, B.J. 2015. Parallel ionoregulatory adjustments underlie phenotypic plasticity and evolution of Drosophila cold tolerance. J. Exp. Biol. 218: 423-432.

Staples, J.F. 2014. Metabolic Suppression in Mammalian Hibernation: The Role of Mitochondria. J. Exp. Biol. 217:2032-2036. doi:10.1242/jeb.092973

Brown, J.C.L. and Staples, J.F. 2014. Substrate-specific suppression of mitochondrial subpopulations in skeletal and cardiac muscle of hibernating thirteen-lined ground squirrels. J. Comp. Physiol B. 184(3):401-414. DOI  - 10.1007/s00360-013-0799-3.

Cooper, A.N., Brown, J.C.L. and Staples, J.F. 2014. Are long chain acyl CoAs responsible for suppression of mitochondrial metabolism in hibernating 13-lined ground squirrels. Comp. Biochem. Physiol. B. 170:50-57. DOI - 10.1016/j.cbpb.2014.02.002

Neimanis, K., Staples, J.F. Hüner, N.P.A. and McDonald, A.E. 2013. Identification, Expression, and Taxonomic Distribution of Alternative Oxidases in Non-Angiosperm Plants. Gene 526:275-286.

Chung, D.C., Szyszka, B., Brown, J.C.L., Hüner, N.P.A. and Staples, J.F. 2013. Changes in the mitochondrial phosphoproteome during mammalian hibernation. Physiol. Genom. 45(10):389-399.

James, R.S., Staples, J.F., Brown, J.C., Tessier, S.N. and Storey, K.B. 2013. The effects of hibernation on the contractile and biochemical properties of skeletal muscles in the thirteen-lined ground squirrel, Ictidomys tridecemlineatus. J. Exp. Biol. 216:2587-2594.

Suri, L.M.N., Cruz, A., McCaig, L., Veldhuizen, R.A.W., Staples, J.F., Possmayer, F. Orgeig, S., Perez-Gil, P. 2013. Adaptations to hibernation in lung surfactant composition of 13-lined ground squirrels influence surfactant lipid phase segregation properties. Biochim. Biophys. Acta. 1828(8):1707-1714.

Brown J.C.L., Chung D.J., Cooper A.N., Staples J.F. 2013. Regulation of succinate-fuelled mitochondrial respiration in liver and skeletal muscle of hibernating thirteen-lined ground squirrels. J. Exp. Biol. 216:1736-1743.

Price, E.R., C. Armstrong, C, Guglielmo, C.G. and Staples, J.F. 2013. Selective mobilization of saturated fatty acids in isolated adipocytes of hibernating thirteen-lined ground squirrels Ictidomys tridecemlineatus. Physiol. Biochem. Zool. 86(2):205-212.

Gallagher, K. and Staples, J.F. 2013. Metabolism of brain cortex and cardiac muscle mitochondria in hibernating thirteen-lined ground squirrels (Ictidomys tridecemlineatus). Physiol. Biochem. Zool. 86(1):1-8.

MacMillan, H.A, Williams, C.M., Staples, J.F. and Sinclair, B.J. 2012. Reestablishment of ion homeostasis during chill-coma recovery in the cricket Gryllus pennsylvanicus. Proc. Nat. Acad. Sci. 109(50):20750-20755.

Staples, J.F. Fast In, Slow Out: Patterns and Mechanisms of Mitochondrial Metabolic Suppression in Mammalian Hibernation. In: Ruf T, Bieber C, Arnold W, Millesi E (eds). 2012. Living in a seasonal world: thermoregulatory and metabolic adaptations. Pp. 377-386. Springer, Heidelberg.

Brown, J, Marshall, K., Staples, J. 2012. Differences in tissue concentrations of hydrogen peroxide in the roots and cotyledons of annual and perennial species of flax (Linum). Botany. 90(10): 1015-1027

Suri L., McCaig L., Picardi, M., Ospina, O., Veldhuizen, R., Staples, J., Possmayer, F., Yao, L., Perez-Gil, J., Orgeig, S. 2012. Adaptation to low body temperature influences pulmonary surfactant composition thereby increasing fluidity while maintaining appropriately ordered membrane structure and surface activity. BBA-Biomembranes. 1818:1581-1589.

MacMillan, H.A, Williams, C.M., Staples, J.F. and Sinclair, B.J. 2012. Metabolism and energy supply below the critical thermal minimum of a chill-susceptible insect. J. Exp. Biol. 215:1366-1372.

Brown, J.C.L., Chung, D.J., Belgrave, K.R. and Staples, J.F. Mitochondrial metabolic suppression and reactive oxygen species production in liver and skeletal muscle of hibernating thirteen-lined ground squirrels. 2012. Am. J. Physiol. 302:R15-R28.

Staples, J.F. Maintaining metabolic balance in mammalian hibernation and daily torpor. 2011. in Nowakowska, A. and M. Caputa (eds.) Hypometabolism: strategies of survival in vertebrates and invertebrates. Research Signpost, Kerala, India. pp. 95-115.

Brown, J.C.L. and Staples, J.F. 2011. Suppression of mitochondrial metabolism during daily torpor and fasting in house mice (Mus musculus) and dwarf Siberian hamsters (Phodopus sungorus): consequences for reactive oxygen species release. Physiol. Biochem. Zool. 84:467-480.

Armstrong C., Thomas, R., Price, E.R., Guglielmo, C. G. and Staples, J. F. 2011. Remodeling mitochondrial membrane during arousal from hibernation. Physiol. Biochem. Zool. 84:438-449.

Chung, D., Lloyd, G.P., Thomas, R.H., Guglielmo, C.G. and Staples, J.F. 2011. Mitochondrial respiration and succinate dehydrogenase are suppressed early during entrance into a hibernation bout, but membrane remodeling is only transient. J. Comp. Physiol. B 181(5):699-711.

Price, E.R., Staples, J.F., Milligan, C.L. and Guglielmo, C.G. 2011. Carnitine palmitoyl transferase activity and whole muscle oxidation rates vary with fatty acid substrate in avian flight muscles. J. Comp. Physiol. B. 181(4):565-573.

Publications 1995-2010

Armstrong, C. and Staples, J.F. 2010. The role of succinate dehydrogenase and oxaloacetate in mitochondrial metabolism during mammalian hibernation and arousal. J. Comp. Physiol. B. 180:775-783.

Brown, J.C.L. and Staples, J.F. 2010. Mitochondrial metabolism during fasting-induced daily torpor in mice (Mus musculus). Biochim. Biophys. Acta-Bioenerg. 1797(4):476-486.

Brown, J.C.L., McClelland, G.B., Faure, P.A., Klaiman, J.M. and Staples,
J.F. Mechanisms of reduced ROS production in long-lived species. 2009. Mech. Ageing Dev. 130:467-476.

McDonald, A.E., Vanlerberghe, G.C. and Staples, J.F. Alternative oxidase in animals: Unique characteristics and taxonomic distribution. 2009. J. Exp. Biol. 212:2627-2634

Staples, J.F. and Buck, L.T. 2009. Matching metabolic supply and demand in energy-stressed animals. Comp. Biochem. Physiol. A. 153:95-105.

Page, M.M., C. Peters, J.F. Staples and Stuart J.A. 2009. Intracellular superoxide dismutases are not globally upregulated during hibernation in the major oxidative tissues in the 13-lined ground squirrel Spermophilus tridecemlineatus. Comp. Biochem. Physiol. A 152:115-122.

Staples J.F. and J.C.L. Brown. 2008. Mitochondrial metabolism in hibernation and daily torpor: a review. J. Comp. Physiol. B. 178:811-827.

Gerson, A.R., J.C.L. Brown, R. Thomas, M.A. Bernards and J.F. Staples.
2008. The effect of dietary polyunsaturates on mitochondrial metabolism in hibernation. J. Exp. Biol. 211: 2689-2699.

Staples, J.F., Kajimura, M., Wood, C.M., Patel, M., Ip, Y.K., and McClelland, G.B. (2008) Enzymatic and mitochondrial responses to 5 months of aerial exposure in the slender lungfish (Protopterus dolloi). J. Fish Biol.73:608-622.

Ellard, J.P., McCudden, C.R. Tanega, C. James, K.A., Ratkovic, S., Staples, J.F., Wagner, G.F. 2007. The respiratory effects of stanniocalcin-1 (STC-1) on intact mitochondria and cells: STC-1 uncouples oxidative phosphorylation and its actions are modulated by nucleotide triphosphates. Mol. Cell. Endocrinol. 264:90-101.

Brown, J.C.L., A.R. Gerson, J.F. Staples. 2007. Mitochondrial metabolism during daily torpor in the dwarf Siberian hamster: the role of active regulated changes and passive thermal effects. Am. J. Physiol. 293:R1833-1845.

Muleme, H. M, Walpole, A. C. and Staples, J. F. 2006. Mitochondrial metabolism in hibernation: Metabolic suppression, temperature effects and substrate preferences. Physiol. Biochem. Zool. 79(3): 474-483.

West, T.G., Donohoe, P.H., Staples, J.F. and Askew, G.N. 2006. The role of skeletal muscle in the hypoxia induced responses of submerged frogs., J. Exp. Biol. 209:1159-1168.

Schaefer, C. D. and Staples, J. F. 2006. Mitochondrial metabolism in mammalian cold acclimation: magnitude and mechanisms of fatty acid induced uncoupling. J. Therm. Biol. 31(4):355-361.

Staples, J. F., Koen, E.L. and Laverty, T.L. 2004. “Futile cycle” enzymes in the flight muscles of North American bumblebees. J. Exp. Biol. 207(5): 749-754.

Staples, J. F., Webber, D. M. and Boutilier, R. G. 2003. Environmental hypoxia does not constrain the diurnal depth distribution of free-swimming Nautilus pompilius. Physiol. Biochem. Zool. 76(5):644-651.

Staples, J. F., Hershkowitz, J. J. and Boutilier, R. G. (2000). Effects of ambient PO2 and temperature on oxygen uptake in Nautilus pompilius. J. Comp. Physiol. B. 170(3):231-236.

Suarez, R. K., Staples, J. F., Lighton, J. R. B. and Mathieu-Costello, O (2000). Mitochondrial function in flying honeybees (Apis mellifera): respiratory chain enzymes and electron flow from complex III to oxygen. J. Exp. Biol. 203:905-911.

Suarez, R. K., Staples, J. F. and Lighton, J. R. B. (1999). Turnover rate of mitochondrial respiratory chain enzymes in flying honeybees (Apis mellifera). J. Exp. Zool. 284(1):1-6.

Staples, J. F. and Hochachka, P. W. (1998). The effect of hibernation status and cold-acclimation on hepatocyte gluconeogenesis in the golden-mantled ground squirrel (Spermophilus lateralis). Can. J. Zool. 76(9):1734-1740.

Hochachka, P. W., McClelland, G.B., Burness, G.P., Staples, J.F. and Suarez, R.K. (1998) Integrating metabolic pathway fluxes with gene-to-enzyme expression rates. Comp. Biochem. Physiol. B. 120(1):17-26.

Staples, J. F. and Suarez, R. K. (1997). Honeybee flight muscle phosphoglucose isomerase: Matching enzyme capacities to flux requirements at a near-equilibrium reaction. J. Exp. Biol. 200:1247-1254.

Staples, J. F. and Hochachka, P. W. (1997). Liver energy metabolism during hibernation in the golden-mantled ground squirrel, Spermophilus lateralis. Can. J. Zool. 74(7):1059-1065.

Suarez, R. K., Staples, J. F., Lighton, J. R. B. and West, T. G. (1997). Relationships between enzymatic flux capacities and metabolic flux rates: Nonequilibrium reactions in muscle glycolysis. Proc. Natl. Acad. Sci. 94:7065-7069.

Staples, J. F., Zapol, W. M., Bloch, K. D., Kawai, N., Val, V. M. F. and Hochachka, P. W. (1995). Nitric oxide responses of air-breathing and water-breathing fish. Am. J. Physiol. 268:  R816-R819.