Dr. John P. Wiebe
Hormonal Regulatory Mechanisms
Recent publications related to our breast cancer research
1. Wiebe JP, Muzia D, Hu J, Szwajcer D, Hill SA & Seachrist JL. 2000. The 4-pregnene and 5alpha-pregnane progesterone metabolites formed in non-tumorous and tumorous breast tissue have opposite effects on breast cell proliferation and adhesion. Cancer Research 60:936-943.
2. Weiler PJ & Wiebe JP. 2000. Plasma membrane receptors for the cancer-regulating progesterone metabolites, 5alpha-pregnane-3,20-dione and 3alpha-hydroxy-4-pregnen-20-one in MCF-7 breast cancer cells. Biochemical and Biophysical Research Communications 272: 731-737.
3. Wiebe JP & Muzia D. 2001. The endogenous progesterone metabolite, 5alpha-pregnene-3,20-dione, decreases cell-substrate attachment, adhesion plaques, vinculin expression, and polymerized F-actin in MCF-7 breast cancer cells. Endocrine 16: 7-14.
4. Wiebe JP & Lewis MJ. 2003. Activity and expression of progesterone metabolizing 5alpha-reductase, 20alpha-hydroxysteroid oxidoreductase and 3alpha(beta)-hydroxysteroid oxidoreductases in tumorigenic (MCF-7, MDA-MB-231, T-47D) and nontumorigenic (MCF-10A) human breast cancer cells. BMC Cancer 2003 3(9) (pp 1-15), (http://www.biomedcentral.com/1471-2407/3/9).
5. Lewis MJ, Wiebe JP & Heathcote JG. 2004. Expression of progesterone metabolizing enzyme genes ( AKR1C1, AKR1C2, AKR1C3, SRD5A1, SRD5A2) is altered in human breast carcinoma. BMC Cancer 4 (27) (pp 1-12) (http://www.biomedcentral.com/1471-2407/4/27).
6. Wiebe JP, Zhang G & Woodford SD. 2004. The progesterone metabolites, 5alpha-dihydroprogesterone (5aP) and 3alpha-hydroxy-4-pregnen-20-one (3aHP), have opposing effects on cell substrate adhesion, amount of polymerized actin and adhesion plaque-associated vinculin in MCF-7, MCF-10A, MDA-MB-231 and T47D breast cell lines. The Endocrine Society 86th Annual Meeting, New Orleans, 2004. Abstract.
7. Wiebe JP, Lewis MJ, Cialacu V, Pawlak KJ & Zhang G. 2005. The role of progesterone metabolites in breast cancer: potential for new diagnostics and therapeutics. Journal of Steroid Biochemistry and Molecular Biology 93: 201-208.
8. Wiebe JP. 2005. Role of progesterone metabolites in mammary cancer. The Journal of Dairy Research 72: 51-57.
9. Pawlak KJ, Zhang G, Wiebe JP. 2005. Membrane 5alpha-pregnane-3,20-dione (5aP) receptors in MCF-7 and MCF-10A breast cancer cells are up-regulated by estradiol and 5aP and down-regulated by the progesterone metabolites, 3alpha-dihydroprogesterone and 20alpha-dihydroprogesterone, with associated changes in cell proliferation and detachment. Journal of Steroid Biochemistry and Molecular Biology 97: 278-288.
10. Wiebe JP, Souter L & Zhang G. 2006. Dutasteride affects progesterone metabolizing enzyme activity/expression in human breast cell lines resulting in suppression of cell proliferation and detachment. Journal of Steroid Biochemistry and Molecular Biology, 100: 129-140.
11. Wiebe JP. 2006. Progesterone metabolites in breast cancer. (Invited Review) Endocrine-Related Cancer. 13: 717-738.
12. Pawlak KJ, Wiebe JP. 2007. Regulation of estrogen receptor (ER) levels in MCF-7 cells by progesterone metabolites. Journal of Steroid Biochemistry and Molecular Biology, 107: 172-1179.
13. Wiebe JP, Beausoleil M, Zhang G, Cialacu V. 2009. Opposing actions of the progesterone metabolites, 5alpha-dihydroprogesterone (5alphaP) and 3alpha-dihydroprogesterone (3alphaHP) on mitosis, apoptosis, and expression of Bcl-2, Bax and p21 in human breast cell lines. Journal of Steroid Biochemistry and Molecular Biology, in press, doi:10.1016/j.jsbmb.2009.11.005.
