Dr. van Huystee's links:
Dr. Robert van Huystee
The Structure and Function of 3 Peroxidase Glycans
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Position: Professor Emeritus Office: Collip Room 112 Phone: 519 661-2111 ext.82640 Fax: 519 661-3935 Email: huystee@uwo.ca |
Research Statement
Peroxidase is a hemo-, calcium-, and glycoprotein. From cultured peanut cells one can obtain large amounts in relatively pure state. The major cell wall isozyme has been isolated as crystals and diffracted so that the position of the heme and 2 moles of calcium have been identified. The calcium plays a major role as a protein stabilizer and the heme is needed for its oxido-reductase function. At present the glycan chains are being studied. One has been isolated and micro-heterogeneity has been found in the complex 11 sugar containing glycan. The use of site-directed mutagenesis, for each of the three glycan binding sites, has shown that the sugars are also needed for folding as well as function. Present efforts are directed towards MALDI-TOF examinations of the glycans so that a complete picture of the molecule may be obtained. In the age of proteomics such appears to be an interesting issue.
